Research Applications of MicroRNA Agomir/Antagomir - PDF Flipbook

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Research Applications of MicroRNA Agomir/Antagomir

Since its discovery in 1993, microRNA (miRNA) has proven its value in biotechnological
research many times over. MicroRNA is a small (approximately 22 nucleotides), endogenous,
non-coding RNA molecule that occurs in animals, plants, and certain viruses. These molecules
have been found to play a hugely important role in the regulation of gene expression after
transcription, and well over one thousand miRNAs can be found in the human genome. They are
believed to influence at least 30 percent of human genes.1

MiRNA inhibitors and mimics promote RNA silencing by cleaving the messenger RNA
(mRNA) strand into separate pieces, destabilizing the mRNA via shortening of the poly(A) tail,
and/or interfering with the translation of mRNA into proteins.2

MiRNA agomir/antagomir synthetic molecules provide biotech researchers with a powerful tool
for their investigations.

About Agomir

Agomir is a chemically modified double-strand miRNA mimic that regulates the expression of
targeted genes. It has a greater cell membrane binding affinity than is found in most miRNA
mimics, which enhances its value as a research tool. By mimicking mature miRNAs after
transfection into cells, agomir effectively up-regulates endogenous miRNA activity. Chemical
modifications made to agomir can significantly enhance its stability in vivo.

About Antagomir

Antagomir is a chemically modified single-strand miRNA antagonist that inhibits the function of
miRNAs by interfering with their ability to pair with target sequences. Their ability to inhibit
activity in specific miRNAs (such as microRNA-21 and microRNA-122) is long-lasting,
potentially remaining effective for weeks. Like agomir, it is noted for its cell membrane binding
affinity. The chemical modification of this miRNA inhibitor also serves to make it relatively
resistant to degradation.

Advantages of MicroRNA Agomir/Antagomir

Owning to its vast importance in gene regulatory networks, miRNA offers enormous potential to
researchers involved in the investigation of cellular processes such as proliferation,
differentiation, and apoptosis. MiRNA research promises to produce breakthroughs in the
treatment of cancer, diabetes, heart disease, and other disorders.3

Agomir/antagomir synthesis services are available to biotech professionals who need these
molecules for research purposes. Their best option is to obtain these services from a reputable
biotechnological company like AcceGen. AcceGen’s agomir/antagomir synthesis services
provide a number of important benefits to researchers:

● Easy to inject – AcceGen's agomir and antagomir can be introduced into an animal via
local or intravenous injection.

● Ready for transfection – These MiRNA mimics and inhibitors will arrive purified.

● Comprehensiveness – AcceGen's agomir/antagomir synthesis services includes all the
human, mouse, & rat miRNAs listed on the microRNA database miRBase (mirbase.org).

● High stability – AcceGen's agomir and antagomir can easily pass through cell
membranes.

● Cost-effectiveness – Short synthesis cycles reduce costs.4

AcceGen's MicroRNA agomir/antagomir database is online and open to the public. More than
5,000 human (hsa-miR-), mouse (mmu-miR-), and rat (rno-miR-) agomir/antagomir miRNAs are
ready for shipping worldwide.

About AcceGen

Founded in 2016, AcceGen is a biotechnological company that develops and distributes cell and
genomic research solutions for professionals and organizations in the pharmaceutical, cell
biology, biotech, and specialty ingredients fields. The company offers a broad array of biotech
resources, including stem cells, primary cells, immortalized cell lines, nucleic acid kits,
transfected stable cell lines, agomir/antagomir synthesis services, and more. AcceGen is
headquartered in Fairfield, New Jersey.

Sources

1. https://www.news-medical.net/life-sciences/What-is-MicroRNA.aspx
2. https://www.accegen.com/recent-posts/microrna-help-us-understand-many-important-

life-processes/
3. https://www.sciencedirect.com/topics/neuroscience/microrna
4. https://www.accegen.com/services/microrna-agomir-antagomir-synthesis/